Model-Based Optimization of Mannitol Production by Using a Sequence of Batch Reactors for a Coupled Bi-Enzymatic Process—A Dynamic Approach

Multi-enzymatic reactions can successfully replace complex chemical syntheses, using milder reaction conditions, and generating less waste. The present model-based analysis compares the performances of several optimally operated Batch Reactors (BR) with those an serial Sequence BRs (SeqBR). In multi-enzymatic systems, SeqBR could be more advantageous flexible, allowing optimization costly enzymes amounts used in each BR series. Exemplification was made for bi-enzymatic reduction D-fructose to mannitol by MDH (mannitol dehydrogenase) NADH cofactor, situ continuous regeneration at expense formate degradation presence FDH (formate dehydrogenase). For such coupled enzymatic engineering evaluations are difficult tasks, because they must account common species’ initial levels, their interaction, dynamics. determination optimal operating modes sole or a turns into multi-objective problem multiple constraints solved every particular system. study presents elements novelty: (i) proof higher (including N-BRs) compared N-number runs (ii) effect criteria on adjustable